NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES

NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES (NICED) ANNUAL REPORT 2001-2002

Particulars	Page No.
1. Study on hospital surveillance	10
1.1. Hospital based surveillance system for diarrhoeal diseases	10, 12
2. Community based studies	13
2.1. A rural community based longitudinal study on diarrhoeagenic Escherichia coli amongst children below 5 years	13
2.2 Impact of zinc supplementation on incidence of diarrhoea and growth pattern among low birth weight infants in an urban slum	14, 15, 16, 17
2.3. A prospective study of gender bias in health care practices with special reference to childhood diarrhoea, acute respiratory infections and protein energy malnutrition	18
3. Clinical studies	19
3.1. Efficacy and safety of a sucrose based hypo-osmolar ORS solution in adults and older children with cholera : a clinical trial	19
3.2. Evaluation of the role of zinc supplementation in the treatment of cholera both in children and adults	20
4. Studies on Vibrio cholerae O1 and O139	21
4.1. Molecular epidemiology of cholera in India	22
4.2. Characterization of rough strains of Vibrio cholerae isolated from diarrhoea patients	23, 24, 25, 26, 27
4.3. Detection and analysis of mutations in gyrA and parC genes associated with fluroquinolone resistance among clinical strains of Vibrio cholerae and Vibrio parahaemolyticus	28
4.4. Study the role of plasmids in adherence mechanism of V.cholerae nonO1, nonO139	28, 29
4.5. Studies on the structure-function relationship of Vibrio cholerae hemolysin (HlyA)	30
4.6. Morphological studies on the flagella of Vibrio cholerae	31
4.7. Molecular regulation of intestinal folate transport under the influence of cholera toxin	33, 34
4.8. Studieson the ststus of baseline serum vibriocidal antibody level against Vibrio cholerae O1 and serogroup O139 in Calcutta community	35, 36
5. Studies on Vibrio cholerae Phages	37
5.1. Evaluation of new phage typing scheme for V.cholerae O1 biotype ElTor strains	33
5.2. Search for newer phages to develop a typing scheme for V.cholerae non-O1 (O139)	37
5.3. Molecular analysis of Vibrio cholerae bacteriophages: cloning and sequencing of Phage DNA	38
6. Studies on Shigella species	43
6.1. Genetic studies on virulence mechanisms of Shigella dysenteriae 1 in relation to vaccine development	43
6.2. Molecular characterization of multi-drug resistant shigella flexneri in kolkata	44
6.3. Antigenic recognition of Shigella dysenteriae outer membrane proteins using human convalescent sera and to evaluate their role in cell-mediated immune response in shigellosis	45
6.4. Immunoregulatory functions of porin of Shigella dysenteriae type 1	46
7. Studies on Escherichia coli	48
7.1. Studies to determine the role of enteroaggregative Escherichia coli for causing diarrhoea with special reference to its virulence properties	48
7.2. Search for Shiga toxin producing Escherichia coli (STEC) including O157:H7 strains in animals products, hospitalized bloody diarrhoea cases and amongst the healthy human population in a defined community	49
7.3. Studies on the binding of Escherichia coli heat-stable enterotoxin to intestinal epithelial cells and brush border membranes of different animals	50, 51
8. Studies on other bacterial enteropathogens	52
8.1. Mode of action of Yersinia enterocolitica heat stable enterotoxin (YSTa) in rat intestinal epithelial cell	52
8.2. Correlation of histology with genotypes of Helicobacter pylori isolated from cases of peptic ulcer, non ulcer dyspepsia,gastric carcinoma and lymphoma	53
8.3. Differentiation of Salmonella enterica serotype Typhi by molecular methods	54
9. Studies on diarrhoeagenic parasites	55
9.1. Studies on electron dense granules of E. histolytica containing collagenase activity	55
9.1. Studies on electron dense granules of E. histolytica containing collagenase activity	56
9.3. Studies on rRNA processing in Giardia lamblia	58, 59, 60, 61
9.4. Detection and characterization of Cryptosporidium parvum by molecular biological tools	62
10. Studies On Viral Enteropathogens	63, 64, 65
10.1. Detection and molecular characterization of rotaviruses	65
10.2. Detection and molecular characterization of human caliciviruses and astrovirus	66, 67
10.3. Molecular characterization of hepatitis C virus	68
11. Investigations Of Outbreaks	69
11.1. Report of investigation of outbreak of unknown fever at Siliguri, West Bengal	69
11.2. Investigation of an outbreak of acute diarrhoeal diseases in a part of district Sarguja in Chhattisgarh	70
11.3. Report of investigation of outbreak of diarrhoeal disease at Chindwara district of Madhya Pradesh and Sarguja district of Chattisgarh states	71
11.4. Investigation of hepatitis outbreak at Rishra municipal area, Hooghly district, West Bengal	72, 73
11.5. Investigation of hepatitis outbreak at Kulti municipal area, Bardhaman District, West Bengal	74, 75
12. Report Of The National HIV Reference Centre	76, 77
13. NICED Project For Prevention Of Emerging Diarrhoeal Diseases	78, 79, 80, 81
14. Activities of Training and Extension Division	82
14.1. Workshops/training Programmes conducted	82
14.1.1. ICMR-NICED Training programme on isolation, identification and antimicrobial resistance monitoring of enteric pathogens	82
14.1.2. Workshop on ethical issues in biomedical research	83
14.1.3. NICED/NICD review cum refresher course for nodal officers of the Eastern States and districts under NSPCD	84
14.1.4. ICMR-WHO workshop on use of Information Technology in Bio-medical Research	84
14.1.5. JICA-NICED training course on molecular epidemiology of diarrheal diseases with special reference to cholera	84
14.1.6. NACO/NICED training course on external quality assessment scheme for HIV testing (2nd phase)	85
14.1.7. Training programme on Immunization Strengthening Project	85
14.1.8. Other Training and Teaching Programmes	85
14.1.9. Guest lectures	86
15. Awards/Honours Received By The Scientists/Students	87
16. (a) Training received by the Staff	89, 88
(b) Training received by the students	89
17. Scientific Conference/Seminar/Meeting Attended By The Scientists/Research Fellows/Staff	90, 91, 100, 101, 102
18. List Of Publications	103, 104, 105
19. Committees of the Institute	106, 107, 108, 109, 110, 111
20. List of the Staff	112, 113, 114, 115, 116