NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES (NICED) ANNUAL REPORT 2002-2003

Particulars  Page No.
1. Study on hospital surveillance

10
1.1. Hospital based surveillance system for diarrhoeal diseases 10
2. Community based studies 13

2.1. A rural community based longitudinal study on diarrhoeagenic

Escherichia coli amongst children below 5 years

 13, 14

2.2. An operational study on effect of zinc supplementation on reduction of diarrhoeal morbidity amongst rural children.

 15, 16

2.3. A study of the impact of albendazole on the nutritional status of children between 2-5 years of age in an urban slum of Kolkata

 17
3. Clinical studies 18

3.1. Efficacy and safety of a sucrose based hypo-osmolar ORS in adults and older children with cholera : a clinical trial 18

 18

3.2. Evaluation of the role of zinc supplementation in the treatment of cholera both in children and adults

 19
4. Studies on Vibrio cholerae 20

4.1. Preferential association of the heat-stable enterotoxin gene (stn) with environmental strains of Vibrio cholerae belonging to the O14 serogroup

 21

4.2. Molecular analsysis of V.cholerae O1 ElTor Ogawa strains isolated from cholera epidemic in Nancowry group of islands, Andaman and Nicobar

 22

4.3. Characterization of rough strains of Vibrio cholerae isolated from diarrhoea patients

 23, 24, 25, 26, 27

4.4. Study the enterotoxicity of Vibrio cholerae non-O1, non-O139 in an in-vitro rat intestinal model

 28, 29, 30, 31

4.5. Studies on the structure-function relationship of Vibrio cholerae hemolysin (HlyA)

 32, 33
4.6. Morphological studies on the flagella of Vibrio cholerae 34, 35, 36

4.7. Molecular regulation of intestinal folate transport under the influence of cholera toxin

 37

4.8. Studies on the status of baseline serum vibriocidal antibody level against Vibrio cholerae O1 and serogroup O139 in Calcutta community

 38

4.9. Sequential studies of Vibrio cholerae 0139 and some of its defined virulence phenotypes on intestinal pathology in rabbit ileal loop model purified protein 40 mg - haemorrhagic and cytotoxic effect

 39
5. Studies on Vibrio cholerae Phages 40

5.1. Further application of phage typing scheme for discriminating strains of Vibrio cholerae O139

 40
5.2. Nationwide screening of phage types of V. cholerae O1 biotype ElTor 41

5.3. Molecular analysis of Vibrio cholerae bacteriophages: cloning and sequencing of phage DNA

 42, 43, 44, 45
6. Studies on Shigella species 46

6.1. Genetic studies on virulence mechanisms of Shigella dysenteriae 1 in relation to vaccine development

 46, 47

6.2. Molecular characterization of multi-drug resistant shigella flexneri in kolkata

48

6.3. Antigenic recognition of Shigella dysenteriae outer membrane proteins using human convalescent sera and to evaluate their role in cell-mediated immune response in shigellosis

 48, 49
6.4. Immunoregulatory functions of porin of Shigella dysenteriae type 1 50
7. Studies on Escherichia coli 51

7.1. Search for Shiga toxin producing Escherichia coli including O157: H7 strains in animals, animal products and hospitalised acute diarrhea cases in Kolkata

51, 52

7.2. Studies on the binding of Escherichia coli heat-stable enterotoxin to intestinal epithelial cells and brush border membranes of different animals

 53

7.3. Antibiotic Resistance, Virulence genes and molecular profiles of Shiga Toxin-Producing Escherichia coli Isolated From Diverse Sources in Calcutta, India

 54, 55, 56, 57

7.4. Studies to determine the role of enteroaggregative Escherichia coli (EAggEC) for causing diarrhoea with reference to its virulence properties

 58
8. Studies on other bacterial enteropathogens 59

8.1. Mode of action of Yersinia enterocolitica heat stable enterotoxin (YSTa) in rat intestinal epithelial cell

 59

8.2. Correlation of histology with genotypes of Helicobacter pylori isolated from cases of peptic ulcer, non ulcer dyspepsia,gastric carcinoma and lymphoma Histologic special strain for Hpy1; modified giemsa, gimenez stain

 60, 61

8.3. Environmental isolates of Aeromonas spp. harboring the cagA gene of Helicobacter pylori

 62, 63, 64, 65, 66

8.4. Differentiation of Salmonella enterica Serotype Typhi by molecular methods

 67
9. Studies on diarrhoeagenic parasites 68

9.1. Studies on electron dense granules of E. histolytica containing collagenase activity

 68, 69

9.2. Studies on multiple genes in Entamoeba histolytica during human collagen type I and Ca2+ interaction : Use of mRNA differential display

70
9.3. Studies on rRNA processing in Giardia lamblia 71, 72, 73

9.4. Detection and characterization of Cryptosporidium parvum by molecular biological tools

 74, 75, 76
10. Studies on viral pathogens 77
10.1. Detection and molecular characterization of rotaviruses 77, 78, 79, 80, 81, 82
10.2. Detection and molecular characterization of human Astrovirus 83, 84, 85, 86
10.3. Detection and molecular characterization of hepatitis C virus 87, 88, 89

10.4. Studies on viral hepatitis among hospitalized cases in Calcutta with special reference to hepatitis E

90
11. Epidemic investigation 91

11.1. Epidemic of Shigella dysenteriae type 1 in Jalpaiguri district of West Bengal

91

11.2. Investigation of an outbreak of bacillary dysentery at Diamond Harbour, North Bengal

 91

11.3. Molecular characterization of Vibrio cholerae strains that caused outbreaks in Nancowry islands of Andaman & Nicobar

92, 93

11.4. Outbreak of acute gastro-enteritis in Howrah Municipality area of Howrah District, West Bengal

 94

11.5. Investigation of an outbreak of hepatitis in the area of Pragatipally of South Dum Dum Municipality 

 95, 96
11.6. Epidemic investigation of unknown fever at West Midnapur District 97, 98, 99, 100, 102
12. HIV/AIDS related works 103
12.1. Report of National HIV Reference Centre 103, 104
12.2. HIV/AIDS epidemiology 105, 106

12.3. Distribution of HIV-1 subtypes in Eastern and Northeastern regions of India

 107, 108

13. NICED - JICA Joint Project for prevention of emerging diarrhoeal diseases

 109, 110, 111, 112
14. Activities of training and extension division 113
14.1. Training programme conducted 113, 114, 115, 116, 117, 118, 119, 120, 121
14.2. Summer training programme 122
14.3. Training of WHO fellows 122

14.4. Supply of Vibrio cholerae 01 and 0139 monoclonal antibodies to different laboratories

  123, 124

15. Scientific conference/seminar/meeting attended by the Scientists/Research Fellows/Staff

 125, 126, 127, 128, 129, 130, 131, 132, 133
16. Training received by the scientists students 134, 135

17. Other activities of the scientists 136

 136, 137, 138, 139
18. Award received by the scientists 140, 141
19 Publications 142, 143, 144, 145, 146
20. Committees of the institute 147, 148, 149, 150
21. List of the Staff 151, 152, 153, 154, 155, 156